ROLE AND MECHANISMS OF ASYMMETRIC CELL DIVISION IN STEM CELL DIFFERENTIATION

Abstract: 

Background: From worms to man, multicellular organisms have evolved two distinct ways for producing cell diversity and tissue specialisation. In one, two initially identical daughter cells follow different fates, because each one encounters a different environmental signal after mitosis. In the other, asymmetric division of the mother cell produces daughter cells intrinsically programmed to follow different developmental fates. Asymmetric cell division (ACD) provides the basis of "œstemness", the unique ability of stem cells to self-renew, while maintaining and materialising their full developmental potential. Yet, the factors and mechanisms that regulate this complex process in mammals remain unknown.

Aim: The main aim of the "Asymmetric Division in Stem Cells" project (ADiSC) is to understand the mechanisms that regulate ACD during embryonic development and stem cell differentiation at both, the molecular and the cellular level. Work packages: The work described below adopts complementary in vitro and in vivo approaches and involves research in four different model systems: human cells, mouse, Drosophila melanogaster and sea urchin.

The specific work packages (WP) will determine the role in ACD during stem cell differentiation of:

  • WP1. TGF?² family signalling
  • WP2. Notch signalling
  • WP3. Geminin and Sox 2 proteins
  • WP4. Transcription factors Coup-TF and Shr2 in ACD
  • WP5. Epigenetic modifications of centromeric and pericentromeric chromatin vi) WP6. Proteome, phosphoproteome and interactome-wide changes.

Expected results: Successful completion of this project is expected to yield fundamental knowledge, allowing the development of improved procedures for the directed differentiation of embryonic stem cells and the regeneration of malfunctioning or destroyed tissues (Regenerative Medicine).

 

ADiSC / MIS: 380249

  

Project info

Acronym:
ADiSC
Scientific Coordinator:
Fotsis Theodore
Research Team 1 Leader:
Fotsis Theodore
Research Team 2 Leader:
Taraviras Stavros
Research Team 3 Leader:
Delidakis Christos

Stats

I.D.:
248
Mis:
380249
Duration (months):
36
Budget:
600 000.00
Diavgeia:
ΑΔΑ: Β41Ρ9-7ΡΝ

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