METABOLOMICS AS A TOOL FOR THE ASSESSMENT OF EMBRYO GROWTH AND VIABILITY IN IN-VITRO FERTILISATION

Abstract: 

Although in-vitro fertilisation (IVF) is widely used worldwide to assist infertile couples, success rates remain low, a fact causing severe physical, emotional and financial stress to couples undergoing such treatment. Low pregnancy rates and high rate of multiple gestations demonstrate the need for more efficient viability assessment. Evidence shows that embryo morphology has limited predictive value whereas embryo biopsy is expensive, complex and practically used only for cases of repeated miscarriage or severe infertility.

New knowledge-based selection tools are needed to allow better embryo selection, increase the success rate and allow single-embryo transfer. Monitoring embryo growth during pregnancy is also challenging. Efficient tools to diagnose unwanted effects such as pre-eclampsia, premature birth, endo-uterus delay and so forth may have great impact in next generation obstetrics.

We propose to investigate the potential of metabolomic profiling as a diagnostic tool for the assessment of embryo viability before implantation in IVF and its growth during pregnancy. Metabolomics may provide new biomarkers at the molecular level to be used as an efficient, economical and non(or less)-invasive tool for these purposes. High resolution Mass spectrometry and Nuclear Magnetic Resonance Spectroscopic analysis of IVF culture medium, maternal blood/urine, amniotic and coelomic fluid (collected from planned examinations embryo genetic tests) will be used in conjunction with advanced bioinformatics to find underlying trends and reveal differentiating biomolecules. These specimens contain a multiplex of metabolites which are very important for the development of the embryo. Mapping these samples is expected to allow useful correlations with the fate of pregnancy.

The final scope is to identity markers or concentration patterns that characterise viable embryos or preterm birth and subsequently develop specific assays for diagnostic/prognostic purposes.

Project info

Acronym:
EMBRYOMETABOLOMICS
Coordinating Institution:
Aristotle University of Thessaloniki
Scientific Coordinator:
Theodoridis Georgios
Research Team 2 Leader:
Athanasiadis Apostolos
Research Team 3 Leader:
Theodoridis Georgios
Research Team 4 Leader:
Biliaderis Konstantinos
Research Team 5 Leader:
Makrydimas Georgios

Stats

I.D.:
347
Mis:
377343
Duration (months):
47
Budget:
600 000.00
Diavgeia:
ΑΔΑ: Β41Κ9-62Ε

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