GENOMIC AND FUNCTIONAL APPROACHES FOR UNDERSTANDING INSECTICIDE RESISTANCE MECHANISMS IN MAJOR AGRICULTURAL PESTS

Abstract: 

The whitefly Bemisia tabaci and the spider mite Tetranychus urticae are among the most damaging agricultural pests world-wide. Their control is largely based on insecticides. However, these pests have evolved resistance to most chemicals, a phenomenon particularly striking in Mediterranean countries, due to climate conditions and heavy use of insecticides. Certain insecticide resistance mechanisms have been elucidated at the molecular level, under the impulse of the increasing availability of insect sequence data and modern technologies, and members of our consortium have contributed to this research.

By using biological material from an important European agricultural area and applying state-of-the art post-genomic/proteomic technologies, we will study the mechanisms by which B. tabaci and T. urticae develop resistance to neonicotinoids and ketoenols, respectively, aiming to provide new tools for managing and overcoming this resistance.

The proposed work includes the following Work Packages:

  1. Bioassays and characterization of resistant strains,
  2. molecular analysis of resistance mechanisms using classical sequencing approaches, microarrays and high-throughput 454 transcriptomics,
  3. RNA interference to confirm the importance of candidate genes in detoxification,
  4. recombinant expression and characterization of proteins associated with resistance and screens for inhibitors and
  5. development of molecular diagnostics to detect biotype and resistance traits in field populations of B. tabaci.

The outputs of the study include, a better understanding of insecticide resistance mechanisms, as well as diagnostic tools and leads for the industry.

Potential beneficiaries include academia, organizations involved in pest management and the industry. In line to the specific call, we have assembled a multi-disciplinary team of highly qualified, motivated researchers with complementary skills and commitment to address the objectives of the proposal.

Project info

Acronym:
IResGR
Coordinating Institution:
University of Crete
Scientific Coordinator:
Vontas John
Research Team 2 Leader:
Iatrou Kostas
Research Team 3 Leader:
Kokkinidis Michalis
Research Team 4 Leader:
Skavdis George

Stats

I.D.:
393
Mis:
377301
Duration (months):
45
Budget:
600 000.00
Diavgeia:
ΑΔΑ: Β4ΛΔ9-4ΣΙ

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