UNDERSTANDING TOLERANCE OF PLANTS TO ABIOTIC STRESSES: THE CROSS-TALK OF POLYAMINE DERIVED-HYDROGEN PEROXIDE, HEAT SHOCK PROTEINS AND POLYPHENOLS IN TOLERANCE OF TRANSGENIC PLANTS TO SALINITY, HEAT AND HEAVY METALS

Abstract: 

Generation of reactive oxygen species (ROS) is nodal point in the response of plant cells to stresses. Oxidation of Polyamines (PAs), small aliphatic polycations, by polyamine oxidases (PAOs) contributes to generation of hydrogen peroxide (H202). In tobacco this apoplastic H202, depending on its titers, signals expression of tolerance genes or the PCD syndrome.

Here we propose to further elucidate in Arabidopsis the action of H202 generated by the 5 peroxisomal and cytosolic AtPAOs to tolerance to salinity, extreme temperatures and heavy metals. We will develop Arabidopsis thaliana transgenics with altered the 5 PAO genes (S-AtPAO1-5), respective mutants (Research Group1, RG1), and Ri-HSP90-5 (RG2) and to use them, along with the existing tobacco S-MPAO, A-MPAO; A-SAMDC ones (Physiol Plant 2008:133:140-56; Plant Cell 2008:20:1708-1724) to study their phenological, molecular, biochemical and ecophysiological responses to abiotic stresses (RG1, RG3, RG4), as well as the potential cross-talk among ŸH202 /HSP and phenolics in the tolerance mechanisms (RG1, RG2, RG3, RG4). All transgenics and mutants will be used in a coordinated manner by the Partners to study their responses to salinity, extreme temperatures and heavy metals (Al, Cd). The plants will be analyzed for endogenous PAs (soluble and conjugated Put, Spd, Spm), ROS generation and localization, PAOs transcripts/PAO activities, antioxidant genes/proteins/activities, HSP90 transcripts, endogenous metal contents and phenolics, in the case of heavy metals. Also, the functional parameters of photosynthetic apparatus, NPQss, Pmax, Ci, PSIo, ETo/RC, SFI, and the conductivity of ATPase in protons will be assessed.

The results are exprected to reveal novel tolerance mechanisms. All collaborating researchers have the expertise, the experience, the infrastructure, the materials and the methods for the successful implementation of this proposal. Also, they fulfill intra-disciplinarity and complimentarity.

 

ABISTOLE / MIS: 377281

 

Project info

Acronym:
ABISTOLE
Coordinating Institution:
University of Crete
Scientific Coordinator:
Roubelakis-Angelakis Kalliopi
Research Team 1 Leader:
Roubelakis-Angelakis Kalliopi
Research Team 2 Leader:
Kotzabasis Kyriakos
Research Team 3 Leader:
Hatzopoulos Polydefkis
Research Team 4 Leader:
Ganis-Spyropoulos Caroline
Research Team 5 Leader:
Constantinidou Helen-Isis

Stats

I.D.:
619
Mis:
377281
Duration (months):
36
Budget:
600 000.00
Diavgeia:
ΑΔΑ: Β4ΛΔ9-6ΚΛ

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