METAGENOMICS OF LIGNOLYTIC MICROORGANISMS - BIOCONVERSION OF PLANT BY-PRODUCTS INTO HIGH-ADDED VALUE PRODUCTS

Abstract: 

Olive-oil mill wastes (OMW) are produced in enormous quantities and are characterized by their high biotoxicity and recalcitrance, mainly attributed to their content in polyphenolics and long-chain fatty acids. Many of OMW's organic constituents are identical or structurally similar to products derived from the enzymatic degradation of lignin by microorganisms.

Currently, the most important applications of lignolytic enzymes produced by cultivated microorganisms are associated with environmental bioremediation and biotreatment technologies, including the degradation and detoxification of notorious pollutants and the bioconversion of a large array of wastes into value-added products. However, since only 1-5% of the known microbial diversity is culturable, the application of modern molecular approaches based on the direct isolation of total microbial DNA from environmental samples could lead to the detection of non-cultivated microorganisms and/or new gene products that can be exploited for their biochemical potential.

The proposal aims to:

  • assess the pertinent biodiversity, molecular phylogeny and ligninolytic genes/gene-products of microbial populations present in OMW and soils receiving OMW for many years vis-a-vis wood-degrading fungi which selectively decompose lignin and its derivatives in natural/forest ecosystems,
  • detect new lignolytic fungi and/or new genes through metagenomics and deep-sequencing,
  • select and modify fungal strains for OMW detoxification applications,
  • produce novel biocatalysts via heterologous gene-expression and over-expression genetically manipulated constructs,
  • detoxify and bioconvert agricultural and forestry residues into edible biomass (mycelium and mushrooms of Pleurotus and Ganoderma spp.) of high nutritional and pharmaceutical value.

Project info

Acronym:
LignoMet
Scientific Coordinator:
Typas Miltos
Research Team 2 Leader:
Zervakis Georgios
Research Team 3 Leader:
Christakopoulos Paul
Research Team 4 Leader:
Antonopoulou Smaragdi

Stats

I.D.:
950
Mis:
377062
Duration (months):
43
Budget:
599 976.00
Diavgeia:
ΑΔΑ: Β4Λ39-ΥΤΟ

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