IDENTIFICATION AND EVALUATION OF NOVEL BIOMARKERS FOR EARLY DIAGNOSIS, PROGNOSIS, AND TREATMENT MONITORING OF BREAST AND PROSTATE CANCERS, THROUGH THE DEVELOPMENT OF HYPERSENSITIVE MOLECULAR METHODOLOGIES

Abstract: 

The identification of new biomarkers is urgently needed for breast and prostate cancers. In this respect, the novel kallikrein-related peptidase (KLKs) genes (KLK7, KLK11, KLK13, KLK15), associated with cancer progression, may serve as valuable molecular tools. Additionally, this proposal will include the study of the biomarker potential of BCL2L12, DDC and RNASEK genes, discovered and cloned by the participants and, based on preliminary results, are implicated in carcinogenesis.

The objectives of this project are the identification and clinical evaluation of novel multilevel (mRNA, microRNA, protein) cancer biomarkers derived from cancer-related genes, through a network of experienced researchers, aiming at the enrichment of early diagnosis, the effective prognosis and treatment monitoring of breast and prostate cancer patients.

This study will also provide reliable and cost-efficient technological platforms for parallel assessment of multiple cancer biomarkers.

Workpackages:

  1. Project coordination and management.
  2. Development of qReal-Time PCR based methodologies.
  3. Development of a novel multianalyte technological platform for the simultaneous measurement of selected genes transcripts expression levels.
  4. Acquisition of a statistically significant tissue and blood sample size from breast and prostate cancer patients, and assessment of the mRNA levels of the gene transcripts under study.
  5. Quantification of the protein isoform levels under study.
  6. Identification and expression analysis of microRNAs (miRNAs) targeting the selected genes. Evaluation of their clinical value.
  7. Study of the effect of chemotherapy on the expression of the genes under study in human breast and prostate cancer cells.
  8. Biostatistical analysis and development of multivariate models.

The results of this study will lead to the utilization of the genes of interest in the development of novel biomarkers for breast, prostate and probably other malignancies.

Project info

Acronym:
BIOPROMO
Scientific Coordinator:
Scorilas Andreas
Research Team 2 Leader:
Zografos George
Research Team 3 Leader:
Christopoulos Theodore

Stats

I.D.:
244
Mis:
377046
Duration (months):
45
Diavgeia:
ΑΔΑ: Β4Λ39-ΒΜ1

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