DEVELOPMENT AND EMPLOYMENT OF MINOSBASED GENETIC AND FUNCTIONAL GENOMIC TECHNOLOGIES IN MODEL ORGANISMS

Abstract: 

Background After having sequenced the genomes of several species, we are faced with the major challenge of understanding the functions of each gene and the genetic, protein-protein and other molecular interactions underlying complex biological processes at the level of cells and organisms.

TE-mediated transgenesis or disruption/modification of gene function caused by random insertion of engineered TEs into the genome can lead to understanding the function of practically any gene of any organism. Minos is a TE with unique advantages for genome-wide functional analysis. It can be mobilized with very high efficiencies in a wide range of species, and inserts into the genome randomly, without any apparent target site preference.

Minos-based methodologies have already been developed by us and others and are being used in a number of organisms including Drosophila and other arthropoda, ascidians, the mouse, and human cells in culture. Because of its unique features, Minos has recently become the TE of choice for genome-wide insertional gene disruption and gene manipulation in Drosophila.

We aim

  • to develop further already available Minos-based technologies for gene/protein trapping in two model organisms (Drosophila melanogaster and Parhyale hawaiiensis) and to employ them in screens for the discovery of genes involved in behavioral habituation and in adhesome structure and function in Drosophila, and the identification of genes activated in limb development and regeneration in Parhyale,
  • to develop Minos-based tools and technologies for heterologous transposition and functional genomic analysis in three important model organisms (Aspergillus nidulans, Arabidopsis thaliana and Caenorhabditis elegans).

The methods that will be developed in Aspergillus and Caenorhabditis will be used for a screen for phenotypes produced by gene silencing and overexpression and a screen designed to identify genes that mediate necrotic cell death, respectively.

Project info

Acronym:
MINOS
Scientific Coordinator:
Savakis Charalambos
Research Team 2 Leader:
Averof Michalis
Research Team 3 Leader:
Diallinas George

Stats

I.D.:
889
Mis:
376898
Duration (months):
42
Budget:
600 000.00
Diavgeia:
ΑΔΑ: Β4ΛΡ9-ΜΑ8

Document Library

News

(07-06-2012) Ένταξη της Πράξης «ΘΑΛΗΣ -  Ε.ΚΕ.ΒΕ. – «ΑΛΕΞΑΝΔΡΟΣ ΦΛΕΜΙΝΓΚ» – Ανάπτυξη τεχνολογίων γονιδιωματικής και γενετικής ανάλυσης με το μεταθετό στοιχείο MINOS και εφαρμογές τους σε οργανισμούς – μοντέλα (ΜΙΝΩΣ)  », με MIS: 376898 στο Επιχειρησιακό Πρόγραμμα «ΕΚΠΑΙΔΕΥΣΗ ΚΑΙ ΔΙΑ ΒΙΟΥ ΜΑΘΗΣΗ» 2007-2013.  -ΑΠ11 (PDF|2,6 MB)